Isolation and Characterization of Stigmasterol and Bis-(5, 7-diacetyl-catechin-4’-α- rhamnopyranoside) from the Stem bark of <i>Neocarya macrophylla</i> (Sabine) Prance (Chrysobalanaceae)

Abstract

Neocarya macrophylla belongs to the Chrysobalanaceae family and is extensively used in folk medicine as an antibacterial, antivenin, antiasthmatic, anticancer, analgesic and anti-inflammatory agent. This study was aimed at isolation and characterization of compounds from the stem bark of Neocarya macrophylla. Pulverized plant material was exhaustively extracted with methanol using maceration method and concentrated invacuo with the aid of rotary evaporator at 40oC to afford a reddish brown crude methanol extract (ME). The methanol extract was successively partitioned into hexane, dichloromethane, ethylacetate, n-butanol and aqueous fractions. Stigmasterol was isolated from the hexane fraction and a catechin glycoside, Bis-(5,7-diacetyl-catechin-4’-α- rhamnopyranoside) was isolated from the ethylacetate soluble fraction using a combination of silica gel column, gel filtration (sephadex LH-20) and preparative thin layer chromatography. The structures of the compounds were established on the basis of chemical tests, spectroscopic analysis and by comparison with reference spectral data.Keywords: Neocarya macrophylla, phytochemical, stigmasterol, Bis-(5, 7-diacetyl-catechin-4’-α-rhamnopyranoside).