Antisickling and antioxidant relevance of twelve ethnomedicinal plants

Abstract

Sickle cell anaemia is the most devastating monogenic disease throughout the world carrying autosomal recessive inheritance. Easily accessible and affordable medicinal plants play the crucial role in the primary treatment of the disease. This study comprises of twelve such medicinal plants having secondary metabolites and their effect on the sickling reversal in vitro, with their antioxidant properties. The antisickling activity of aqueous extracts of plants was calculated at different concentrations (250, 500, 1000 μg/ml). Fifty sickled and fifty normal subjects were involved with their written consents. The spectrophotometrical analysis of antioxidants were determined enzymatically and non-enzymatically at different concentrations. All the plants were found capable of inhibiting RBC hemolysis and sickling but S8 (M. charantia) extract recorded prevention from the hemolysis and antisickling activity at 250 μg/ml and also possess antioxidants. Apart from S8 extract, S12 (S. bicolor), S2 (A. sativum) and S6 (C. sinensis) have also recorded higher antisickling activity and extracts of S2 (A.sativum), S3 (A. occidentale), S4 (C. papaya), S5 (C. cajan), S6 (C. sinensis), S7 (E. caryophyllata), S8 (M. charantia) and S9 (P. guajava) recorded strong antioxidant activities enzymatically and non-enzymatically, thus bestowed promising antioxidant and in vitro antisickling effects.