ANTISEPTIC PROPERTIES OF CITRATE BUFFER

Abstract

The antiseptic properties of endodontic materials are used for microbial control in root canals. Decrease in microfloria is known as one of key demands for root canal therapy. The aim of this work is to study the sensitivity of reference strains of microorganisms to citrate buffer and its combination with the antibiotic «Amoxiclav». We used the museum strains of E. coli ATCC 25922, S. aureus ATCC 25923, S. epidermidis ATCC 14990, E. faecalis ATCC 29212, M. lysodeicus ATCC 4698 and C. albicans ATCC10231 to solve this task. The disk-diffusion method for assessing the sensitivity of standard strains of microorganisms was used. We used chlorhexidine digluconate 0.05% as a control. Citrate buffer has high antibacterial activity. The combination of citrate buffer with antibiotic increases the sensitivity of all reference strains. E. coli ATCC 25922 demonstrates a moderate sensitivity to citrate buffer. This combination with an antibiotic increases the antibacterial effect in 2.3 times (p < 0.05). The sensitivity of enterococci increases towards the direction of chlorhexidine – citrate buffer – citrate buffer + «Amoxiclav». When comparing the results obtained using citrate buffer, its combination with antibiotic and chlorhexidine as a control, we should stress on the lower activity of chlorhexidine to strains of transient and normal oral microflora, which are E. coli ATCC 25922 and M. lysodeisticus ATCC 4698. On the contrary, we have revealed more pronounced action of experimental compositions against strains of pathogenic and opportunistic microorganisms, staphylococci, enterococci and yeast-like fungi that points out the advantage of citrate buffer and its combination with antibiotic «Amoxiclav» in terms of their use in the treatment of complicated caries.