Abstract
Kolaviron (KV), a biflavonoid from Garcinia kola seeds has been reported to possess anti‐inflammatory, antioxidant, hepatoprotective, cardioprotective, nephroprotective and other arrays of chemopreventive capabilities. In this study, the antiproliferative potential of KV was investigated on Vascular Smooth Muscle Cells (VSMCs) in and A549 cancer cell vitro. Lipopolysaccharide (LPS) at 100µg/ml, 1 nm of Angiotensin II (10‐8) and 1 µM of Angiotensin II (10‐6) were used as mitogens to induce cell proliferation and 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was used to measure cell viability. Treatment of VSMCs with Angiotensin II (10‐8), 1 µM of Angiotensin II (10‐6) and LPS significantly enhanced proliferation of VSMCs. Also, treatment of VSMCs with LPS caused significant (p<0.05) increase in nitric oxide (NO) production. Furthermore, excessive NO production was significantly reduced following treatment with KV (25, 50 & 100 µg/ml) respectively. The mechanism of action of KV might be through its anti‐inflammatory and antioxidant capacities. Taken together, KV mitigates Angiotensin II and LPS induced cell proliferation, NO production, inhibited cyclic adenosine monophosphate (cAMP) and induced cytotoxicity in A549 cancer cell proliferation.
Published Version
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