Abstract
Purpose : To determine the phenolic composition and antiproliferative activity of 16 different extracts (hexane, dichloromethane, methanol and water) obtained from Bellis perennis , Convolvulus galaticus , Trifolium pannonicum and Lysimachia vulgaris on human breast cancer (MCF-7) and human hepatocellular carcinoma (HepG2/C3A) cell lines. Methods : The aerial parts of the plants were successively extracted with hexane, dichloromethane, methanol and water using a Soxhlet apparatus. The phenolic content of the plants were determined by plants by high performance liquid chromatography (HPLC) while their antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, a yellow tetrazole (MTT) assay. Results : Among the tested extracts, the methanol extract of B. perennis showed the best antiproliferative activity against MCF-7 cell line with IC 50 (inhibiting 50 % of cell growth) value of 71.6 μg/mL. Furthermore, the dichloromethane extract of C. galaticus showed the best anti-proliferative activity against HepG2/C3A cell line with IC50 of 57.3 μg/mL. The HPLC data for the plant extracts showed the presence of the following phenolic compounds: gallic acid monohydrate, caffeic acid, rutin hydrate, luteolin-7-O-β-D glucoside, kaempferol, myricetin, quercetin, coumarin and apigenin. Conclusion : The findings of this study indicate that there is some justification for the use of B. perennis and C. galaticus as traditional anticancer medicinal herbs. Keywords : Bellis perennis, Convolvulus galaticus, Trifolium pannonicum subsp. elongatum, Lysimachia vulgaris , MCF-7, HepG2/C3A, Phenolics, Breast cancer, Antiproliferative
Highlights
IntroductionBellis perennis L. (common daisy) is a herbaceous perennial herb in the family Asteraceae [1]
Bellis perennis L. is a herbaceous perennial herb in the family Asteraceae [1]
The present study aims, for the first time to the best of our knowledge, to evaluate the antiproliferative properties of B. perennis, C. galaticus, T. pannonicum subsp. elongatum and L. vulgaris extracts against human hepotocellular carcinoma (HepG2/C3A) cell and human breast cancer (MCF-7) cell lines
Summary
Bellis perennis L. (common daisy) is a herbaceous perennial herb in the family Asteraceae [1]. (common daisy) is a herbaceous perennial herb in the family Asteraceae [1]. Common daisy is known as a traditional wound herb [2,3] and it had been used for the treatment of bruises, broken bones [4], sore throat [5], headache [6], common cold [7], rheumatism, inflammation and infections of the upper respiratory tract in traditional medicine [8]. Antibacterial [11], antifungal [11,14], antioxidant [8,11], wound healing activity [2], anxiolytic properties [15], in vitro regeneration protocol [13], antitumor activity [16] and cytotoxic activity against HL-60 human promyelocytic leukemia cells [17] of B. perennis has been investigated. Convolvulus galaticus Rostan ex Choisy (Grizzle bindweed) is an endemic, perennial herb in the family Convolvulaceae. Antibacterial [20,21], antitumor [21] and anticancer activities [22] of C. galaticus have been reported
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