Antiproliferative activity <i>in vitro</i> of <i>Trichinella spiralis</i> larval extracts depending on the infection stage following experimental infection of rats

Abstract

Relevance.The aim is evaluation in vitro antiproliferative activity of T. spiralis larvae extracts obtained from experimentally infected rats, depending on the duration of infection.Methods. The experiment was carried out on outbred male rats infected with T. spiralis larvae at the rate of 10 larvae per 1 g of body weight. The antiproliferative activity of larval extracts obtained 40 days, 3 and 12 months after infection was determined on tumor cells of the A549 line (human lung carcinoma) using an MTT test during cultivation for 24, 48 and 72 hours in concentrations from 32 to 1000 micrograms/ml. Antiproliferative activity was expressed as % inhibition and IC50.Results. There was direct dependence of Trichinella extract antiproliferative effects on time of cultivation with A549 cells and protein concentration in extracts. At all stages of invasion, the antiproliferative activity of the extract increased from 24 to 72 hours. at a protein concentration of 250 to 1000 mcg/ml. The extract antiproliferative activity expressed in tumor cell % inhibition and IC50 increased from day 40 to month 12. The most intense dynamics was observed between day 40 and month 3. The possible reason of enhancement in extract antiproliferative effect at months 3 and 12 is alteration in quantitative and qualitative composition of proteins comprised the extracts.