Abstract
Native berries of Jeju in Korea, Empetrum nigrum var. japonicum and Rubus buergeri Miq., were extracted each fruit or leaves with stem in 80% ethanol to investigate the antioxidant activities. Total contents of flavonoids in leaves and fruit of Empetrum nigrum were 7.33 ± 0.02 mg and 0.44 ± 0.01 mg in 1 g of each plant part, respectively. Total amounts of phenolic compounds in leaves were higher than the fruit for both berries. In 1,1‐diphenyl‐2‐pycryl‐hydrazyl (DPPH) free radical scavenging and superoxide dismutase(SOD)‐like enzyme assay, all extracts showed strong antioxidative effects. Protective effects against H2O2‐induced cow pulmonary artery endothelium(CPAE) cell cytotoxicity were analyzed. CPAE viability was greatly reduced and the survival rate was about 37% when the cells were treated with 1mM H2O2 for 24h. The viability of these H2O2‐treated cells was dramatically improved by preincubation with leaf extracts of both berries. The extracts also inhibited lipid peroxidation in human LDL, measured as the reduction in malondialdehyde(MDA) production. When human LDL was incubated with 100 μg/mL leaf extracts of winter strawberry or crowberry, the value was 1 μM and 1.63 μM of MDA, respectively. This is about 26 times lower than the untreated sample (27.5 μM). Results suggested that Korean native species E. nigrum could be used as a functional material for drinks because of its property of natural antioxidant.
Published Version
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