Antioxidative and Pharmacological Effects of Silymarin

Abstract

Event Abstract Back to Event Antioxidative and Pharmacological Effects of Silymarin Raskovic Aleksandar1*, Stilinovic Nebojsa1, Mikov Momir1, Samojlik Isidora1 and Milijasevic Boris1 1 University of Novi Sad, Department of Pharmacology, Toxicology & Clinical Pharmacology, Serbia Introduction. The present study aimed to investigate the effects of silymarin and doxorubicin on the liver and myocardium function in rats. Methods. White laboratory rats of Wistar type were used in this experiment. They were treated with saline, 1ml/kg, orally (KN, n=8), olive oil 1ml/kg, orally (KU, n=8), silymarin, 60mg/kg, orally, every day, (S, n=8), with doxorubicin, 1,66mg/kg, i.p., every second day (D, n=8), and with the combination of silymarin and doxorubicin in stated doses. After the treatment the animals were anesthetized with urethane at a dosage of 0.75 g/kg i.p., and administered with continuous infusion of lidocaine through the infusion pump. During the lidocaine infusion the EKG of the treated animals was observed. The animals were sacrificed by cardiopunction. Samples of serum were taken in order to determine the transaminase activity, whereas the activity of the enzymes, the indicators of the oxidative stress was determined in liver homogenate. Results.The results of our research showed significant body weight reduction in the group treated with doxorubicin in comparison with the control group and the group treated with the combination of silymarin and doxorubicin (- 27 g, - 15 g). Compared with the control group, the concentration of lidocaine required to cause bradycardia was significantly higher in the group treated with doxorubicin (26,33:15,36, p<0.01). Silymarin administration caused the decrease of the prooxidative parameters: lipide peroxidation (2,25:0,28 p<0,005), catalase activity (5,98: 0,40, p<0,05) and glutathione peroxidase activity (5,30:0,45, p<0,005). Glutathione concentrations were not significantly changed in neither one of the experimental groups. Silymarin prevented the doxorubicin induced increase in aspartate aminotransferase activity (349.5:279.5, p<0.05). Conclusion. Silymarin administration significantly reduces the loss in body weight and prevents the increase of the myocardium excitability in rats treated with doxorubicin. Prooxidative activity of doxorubicin is significantly lower when it is administered in combination with silymarin. Keywords: Silymarin, pharmacokinetics, Liver, Myocardium Conference: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010. Presentation Type: Oral Topic: Xenobiotic toxicity Citation: Aleksandar R, Nebojsa S, Momir M, Isidora S and Boris M (2010). Antioxidative and Pharmacological Effects of Silymarin. Front. Pharmacol. Conference Abstract: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010. doi: 10.3389/conf.fphar.2010.60.00098 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Oct 2010; Published Online: 04 Nov 2010. * Correspondence: Dr. Raskovic Aleksandar, University of Novi Sad, Department of Pharmacology, Toxicology & Clinical Pharmacology, Republic of Serbia, Serbia, araskovic@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Raskovic Aleksandar Stilinovic Nebojsa Mikov Momir Samojlik Isidora Milijasevic Boris Google Raskovic Aleksandar Stilinovic Nebojsa Mikov Momir Samojlik Isidora Milijasevic Boris Google Scholar Raskovic Aleksandar Stilinovic Nebojsa Mikov Momir Samojlik Isidora Milijasevic Boris PubMed Raskovic Aleksandar Stilinovic Nebojsa Mikov Momir Samojlik Isidora Milijasevic Boris Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.