Abstract
Quercetin is one of the most abundant dietary flavonoids widely present in many fruits and vegetables. Previous in vitro studies has shown that quercetin acts as an antioxidant and anti-inflammatory agent and it has potent anticarcinogenic properties as an apoptosis inducer. In this study we examined apoptotic effects of quercetin on the K562 erythroleukemia cell line. K562 cells were induced to undergo apoptosis by hydrogen peroxide. Cell viability and apoptosis level were assessed by annexin V and PI staining methods using flow cytometry. Viability of K562 cells was increased by low dose of quercetin (5-100 μM) for 3 hours. High doses of quercetin proved toxic (100-500 μM, 24 hours) and resulted in decrease of K562 cell viability as expected (P<0.01). As to results, 100 μM quercetin was defined as a protective dose. Also, K562 cell apoptosis due to hydrogen peroxide was decreased in a dose dependent manner. As indicated in previous studies, reduction of superoxides by free radical scavengers like quercetin could be beneficial for prevention of cancer but consumption of such flavonoids during cancer treatment may weaken effects of chemotherapeutics and radiotherapy. Especially cancer patients should be carefully considered for traditional phytotherapy during cancer treatment, which can lead to controversial results.
Highlights
Reactive oxygen species (ROS) are highly reactive molecules generated predominantly during cellular respiration and normal metabolism
As indicated in previous studies, reduction of superoxides by free radical scavengers like quercetin could be beneficial for prevention of cancer but consumption of such flavonoids during cancer treatment may weaken effects of chemotherapeutics and radiotherapy
Cancer patients should be carefully considered for traditional phytotherapy during cancer treatment, which can lead to controversial results
Summary
Reactive oxygen species (ROS) are highly reactive molecules generated predominantly during cellular respiration and normal metabolism. These side products can damage many biological molecules; they can change protein functions, damage DNA material and cause lipid peroxidation of cell membranes (Kulbacka et al, 2009). The reducing environment inside cells helps to prevent free radical mediated damage. What will happen if cancer patient takes artificial herbal sourced antioxidants such as quercetin which is abundantly present in many herbs, fruits and grains. Will it facilitate anticancer therapy or not?. K562 erythroleukemia cell line was induced to apoptosis by hydrogen peroxide and effect of quercetin over the apoptosis and viability of K562 cells was observed with flow cytometer
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