Abstract
The metabolic response of prohexadione-Ca (Pro-Ca) treated cucumber (Cucumis sativus L.) leaves to Pseudomonas syringae pv. lachrymans (Psl) was analyzed at the local and systemic levels. The activities of antioxidant enzymes such as ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POX) utilizing guaiacol and syringaldazine as well as lipid peroxide level were studied. Observed changes in these parameters were compared to the response induced by benzotiadiazole (BTH) treatment after Psl infection. Cucumber plants pretreatment (priming) with Pro-Ca similarly to BTH priming enhanced the defense response of cucumber leaves to subsequent bacterial infection as shown by the suppression of disease development in comparison to the untreated plants. Pro-Ca priming changed the examined parameters in both pathogen-inoculated leaves and systemic (uninfected) ones depending on Pro-Ca concentration used. However, these changes were pronounced especially in the direct inoculated leaves. In the systemic leaves Pro-Ca pretreatment induced different changes profile in examined parameters than BTH.Obtained results suggested that Pro-Ca as BTH affect the pro-antioxidative metabolism under Psl infection conditions but in a different way. BTH induced mainly APX while Pro-Ca APX and CAT. Strong influence of Pro-Ca on both examined types of POX was also found. The POX utilizing syringaldazine activity indicted that in all examined variants Psl infection affected the lignifications of cell wall. Although the physiological significance of examined pro-antioxidative reactions at the local and systemic levels remains to be elucidated, the presented results support for the first time the protective role of Pro-Ca in defense response of cucumber against P. syringae pv. lachrymans.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.