Antioxidant profile of chicken bile extract and correlation with chemical content

Abstract

Component in chicken bile may be similar to bear bile, which contains an immunostimulant agent ursodeoxycholic acid and related to antioxidant effect. The purposes of this study were to evaluate the antioxidant potential of chicken bile extract by the DPPH method, analyze the correlation between total phenolic and flavonoid content with IC50 of DPPH scavenging activities. Chicken bile was cut and dried using freeze dryer, then extracted by reflux using different polarity solvents. A rotary evaporator was used to concentrate the extracts. Calculation of total flavonoid and phenolic content, antioxidant potential using DPPH assay were carried out by UV-visible spectrophotometry, and Pearson’s method was used to analyze their correlation. IC50 of DPPH of ethanolic chicken bile extract was 46.64 µg/ml, while ethyl acetate and n-hexane extracts were 69.99 µg/ml and IC50 71.65 µg/ml, respectively. The highest phenolic content and flavonoid content were given by ethanolic chicken bile extract. The total phenolic and flavonoid content in chicken bile extracts had a significant and negative correlation with IC50 of DPPH. All chicken bile extracts are potential antioxidants by DPPH assay. Phenolic and flavonoid compounds in chicken bile extracts contributed together in antioxidant capacity by DPPH assay.