Abstract
Lyophilized aqueous extracts of four underutilized fruit species namely Diospyros discolor (Velvet apple), Pouteria campechiana (Lavulu/Canistel), Phylanthus acidus (Mal-Nelli/Star gooseberry) and Phyllanthus emblica (Nelli/Indian gooseberry) were investigated for the antioxidant potential (AP) by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay and ferrous reducing antioxidant power (FRAP) assay. Total phenolic content (TPC) and total monomeric anthocyanin content (TMAC) were determined by Folin-Ciocalteu’s colorimetric assay and pH differential method, respectively. Vitamin C (VitC) content of fresh fruit was evaluated titrimertically and expressed as mg of ascorbic acid in 100 g of fresh weight (FW). The TPC and TMAC were expressed as mg of gallic acid equivalents (GAE)/100g FW and mg of cyanodin-3-glucoside (C3G)/100g FW. The measured parameters differed significantly among four fruit species. The values ranged between 84.42 – 1939.70 mg GAE/100g FW, 10.41 – 55.64 mg C3G/100g FW, 0.067 – 310.63 mg FW/ml, 9 – 81.29%, 238.25 – 2891.57 Fe2+μmol/100g FW and 17.12 – 523.14 mg/100g FW for TPC, TMAC, IC50, RSA, FRAP and VitC, respectively. Phyllanthus emblica possessed highest values in all parameters while Phyllanthus acidus showed the lowest except in TPC. The lowest TPC was observed in Diospyros discolor. The extract of Pouteria campechiana also showed considerable amount of TPC (640 mg C3G/100g FW), RSA (76%) and VitC of 53 mg/ 100g. The results revealed that these underutilized fruit crops can be used as sources of natural antioxidants and vitamin C.
Highlights
Reactive oxygen species (ROS) are considered as harmful intermediates produced during oxygen metabolism in biological systems
The results revealed that these underutilized fruit crops can be used as sources of natural antioxidants and vitamin C
Vitamin E considered as a chain breaking antioxidant and reported that regular intakes are correlated with a reduced risk of cardiovascular diseases (Flome and Traber, 1999) these plant extracts can be used as potential candidates to isolate natural antioxidants.Wide varieties of fruit crop species are available in Sri Lanka
Summary
Reactive oxygen species (ROS) are considered as harmful intermediates produced during oxygen metabolism in biological systems. Vitamin C is known to have many biological functions in collagen formation, absorption of inorganic iron, reduction of plasma cholesterol level, inhibition of nitrosoamine formation, enhancement of the immune system and reaction with singlet oxygen and other free radicals (Lee and Kader, 2000). Both vitamin A and E are lipid soluble. Their nutritive, medicinal and therapeutic values are little known to date
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