Antioxidant, physiological and biochemical responses of drought susceptible and drought tolerant mustard (Brassica juncea L) genotypes to rhizobacterial inoculation under water deficit stress

Abstract

Response of drought susceptible (DS) genotype Pusa Karishma LES-39 and drought tolerant (DT) mustard genotype NPJ-124, to rhizobacterial inoculation under water deficit stress, was compared in the present study to determine the influence of inoculants on biochemical and physiological attributes of these two different genotypes. Inoculation was observed to improve root and shoot dry weight in both the genotypes, although better results were observed in the DS genotype. There was variation in the response of the two genotypes to rhizobacterial inoculation, under water deficit stress. Significant improvement in most of the physiological and biochemical parameters including antioxidative enzyme activities of the DS genotype; with concomitant decrease in starch content, accumulation of H2O2 and lipid peroxidation upon inoculation of rhizobacteria was observed. In contrast, there was improvement in only few physiological and biochemical parameters in the DT genotype in response to inoculation with rhizobacteria. There was significant increase in catalase enzyme activity along with concomitant decrease in lipid peroxidation. Thus, drought susceptibility of the mustard genotypes, NPJ-124 and Pusa Karishma LES-39, determined their physiological, biochemical and antioxidative responses to rhizobacterial inoculation under water deficit stress. Expression of drought stress responsive genes belonging to ABA-dependent (RD20 and RD26) and ABA-independent (DREB2 and DREB1-2) pathways was studied in the DS genotype. Expression of DREB2 and DREB1-2 genes was considerably enhanced due to inoculation under water deficit stress; indicating that in Bacillus-mediated priming for drought stress tolerance, in this genotype, ABA-independent pathway probably played key role in enhancing tolerance to drought stress.