Antioxidant of the putat air (barringtonia racemosa) as a herbal remedy to improve sperm quality due to cigarette smoke in rats

Abstract

Background The objective of this study is to analyse the antioxidant in putat air (barringtonia racemosa) as an herbal to improve sperm quality due to cigarette smoke. Methods An experimental study was conducted at animal laboratory, Faculty Veteriner University of Syiah Kuala, Banda Aceh, Indonesia. This study were analyed by one-way ANOVA. A total of 30 male rats (Rattus norvegicus) were divided into 5 treatment groups. Group 1 as a negative control (N) were not exposed to cigarette smoke. Group 2 as a positive control (P) were exposed to cigarette smoke. Group 3 as treatment 1 (P1) were exposed to cigarette smoke and 100 mg/kg BW/day of the barringtonia racemosa seed extract. Group 4 as treatment 2 (P2) were exposed to cigarette smoke and given 150mg/kg BW/day of the barringtonia racemosa seed extract. Group 5 as treatment 3 (P3) were exposed to cigarette smoke and given 200 mg/kg BW/day of the barringtonia racemosa seed extract. The cigarette smoke exposure was carried out in a smoking chamber for a total of 1 hour/rat, and the barringtonia racemosa seed extract is given orally once a day for 30 days. Results This study shows that the sperm quality improved after administration of barringtonia racemosa seed extract in the treatment group (P1-3) compared with the positive control group (P) with statistically significant (p <0.05). Further analysis with the Duncan test showed that administration of barringtonia racemosa seed extract at a dose of 150 mg/kgBW/day (P2) had a statistically significant improvement in sperm quality (P <0.05) compared with a dose of 100 mg/kg/day (P1) and 200 mg/kgBW/day (P3) Conclusions Antioxidants from barringtonia racemosa can improve sperm quality due to cigarette smoke.