Antioxidant of the putat air (barringtonia racemosa) as a herbal remedy to improve sperm quality due to cigarette smoke in rats

Abstract

Background: The objective of this study is to analyse the antioxidant in putat air (barringtonia racemosa) as an herbal to improve sperm quality due to cigarette smoke. Methods: An experimental study was conducted at animal laboratory, Faculty Veteriner University of Syiah Kuala, Banda Aceh, Indonesia. A total of 30 male rats (Rattus norvegicus) were divided into 5 treatment groups. Group 1 as a negative control (N) were not exposed to cigarette smoke. Group 2 as a positive control (P) were exposed to cigarette smoke. Group 3 as treatment 1 (P1) were exposed to cigarette smoke and 100 mg/kg BW/day of the barringtonia racemosa seed extract. Group 4 as treatment 2 (P2) were exposed to cigarette smoke and given 150mg/kg BW/day of the barringtonia racemosa seed extract. Group 5 as treatment 3 (P3) were exposed to cigarette smoke and given 200 mg/kg BW/day of the barringtonia racemosa seed extract. The cigarette smoke exposure was carried out in a smoking chamber for a total of 1 hour/rat, and the barringtonia racemosa seed extract is given orally once a day for 30 days. Results: The results of this study were analyzed by one-way ANOVA showed significant differences in the quality of the sperm group that received cigarette smoke exposure (p <0.05) compared to the administration of the barringtonia racemosa. Improved sperm quality was seen better with the administration of barringtonia racemosa 150 kg/kg BW/day compared with 100 and 200 kg. Conclusions: Antioxidants from barringtonia racemosa can improve sperm quality due to cigarette smoke.