Abstract
The antioxidant function and metabolic profiles in mice after dietary supplementation with methionine were investigated. The results showed that methionine supplementation enhanced liver GSH-Px activity and upregulated Gpx1 expression in the liver and SOD1 and Gpx4 expressions in the jejunum. Nrf2/Keap1 is involved in oxidative stress, and the western blotting data exhibited that dietary methionine markedly increased Keap1 abundance, while failed to influence the Nrf2 signal. Metabolomics investigation showed that methionine administration increased 2-hydroxypyridine, salicin, and asparagine and reduced D-Talose, maltose, aminoisobutyric acid, and inosine 5'-monophosphate in the liver, which are widely reported to involve in oxidative stress, lipid metabolism, and nucleotides generation. In conclusion, our study provides insights into antioxidant function and liver metabolic profiles in response to dietary supplementation with methionine.
Highlights
Methionine is an essential amino acid and mainly contributes to protein and S-adenosylmethionine (SAM) synthesis in the liver
Gas chromatography-mass spectrometry (GC-MS) was performed to investigate the liver metabolic profile after dietary methionine, and the results showed that 7 metabolites were altered and these metabolites are involved in oxidative stress, lipid metabolism, and nucleotides generation
Methionine has been reported to involve in lipid metabolism [27], translational capacity [28], autophagy [29], and maintenance and differentiation of pluripotent stem cells [30]
Summary
Methionine is an essential amino acid and mainly contributes to protein and S-adenosylmethionine (SAM) synthesis in the liver. Methionine has been widely demonstrated to involve in oxidative stress via the transsulfuration pathway for cysteine generation [6]. Cysteine further corresponds for glutathione (GSH) production [6], which is a major thiol group and contributes to antioxidant function against free radical species [7,8,9,10]. Gas chromatography-mass spectrometry (GC-MS) was performed to investigate the liver metabolic profile after dietary methionine, and the results showed that 7 metabolites (i.e., hydroxypyridine, salicin, asparagine, D-talose, maltose, aminoisobutyric acid, and inosine 5’-monophosphate) were altered and these metabolites are involved in oxidative stress, lipid metabolism, and nucleotides generation
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