Abstract
The essential oils of different parts of Platycladus orientalis (Cupressuceae) were examined for their potential antioxidant activity. The compositions of the essential oils of the plant were studied qualitatively and quantitatively by GC and GC-MS. The main components of the essential oils as well as positive controls were subjected to antioxidant testing. Different methods of, rapid TLC screening, diphenylpicrylhydrazyl (DPPH) assay, desoxyribose assay, assay for site-specific reaction and non-enzymatic lipid peroxidation were used to evaluate the antioxidant activity of the oils and various Positive controls. Positive controls were quercetin, vitamin E, vitamin C, dimethylsulfoxide (DMSO) and buthylhydroxytoluene (BHT). a-Pinene, ß-pinene, sabinene, limonene, À-3-carene and terpinolene were the major constituents of these essential oils. The tested compounds mainly showed antioxidant activity when two different rapid TLC screening methods used to evaluation the antioxidants activity. The abilities of the volatile oils to act as nonspecific donors for hydrogen atoms were checked in the diphenylpicrylhydrazyl, DPPH, assay. In this method the strongest effects were shown by the oil of leaves of P. orientalis. In deoxyribose degradation assay, most of the tested compounds showed some antioxidant activity. In non-enzymatic lipid peroxidation test, the oil obtained from leaves of P. orientalis showed strongest antioxidant activity. The results of the study indicate that the essential oils extracted from different parts of P. orientalis, main components of the oils and positive controls show different antioxidant activity when they tested by different antioxidant methods.
Published Version
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