Antioxidant Capacity Of Mango Peel Extract (Mangifera Indica) On Oxidative Stress Induced By Strenuous Exercise

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Strenuous exercise (SE) is a trigger for oxidative stress (OxS) associated with severe tissue damage, fatigue and the development of chronic degenerative diseases. PURPOSE: To evaluate the antioxidant capacity (AOxC) of mango peel extract (MPE) on OxS associated with SE in rats. METHODS: An animal bioassay, approved by bioethics committee, which consisted in an acute SE session performed, or not, after a sub-chronic (1 month) supplementation was executed. The treatments applied were: A) Standard diet (CTRL); B) Standard diet + gallic acid (GA); C) Standard diet + MPE (SMPE). The plasma OxS markers were quantified by Malondialdehyde (MDA) and protein carbonyls (PC). Creatine kinase (CK) and transaminases (ALT & AST) were evaluated as tissue damage markers. Finally, the plasma AOxC was evaluated by ferric reducing antioxidant power (FRAP) assay. RESULTS: The bioassay results are depicted in Table 1. The SE raised PC but no MDA plasma concentrations, on all treatments, also increase CK and AST. Otherwise as response to SE, FRAP activity on plasma was boosted on GA and SMPE. Preliminary phytochemical analysis on MPE presented a total phenolic compound content of 77±3 mg Gallic Acid Eq•g-1 dry weight and 11±0 mg Catechin Eq•g-1 dry weight of total flavonoids. CONCLUSION: The MPE has a high concentration of phenolic compounds and its administration improves the plasma AOxC, but it was not able to inhibit the effects of tissue damage or OxS associated with SE. Also, there was found that MPE has a prooxidative effect by altering the levels of protein oxidation after SE.Table 1: Antioxidant capacity after the bioassay.