Abstract
The aim of this study was to assess the anti-oxidant, astringent, and anti-aging effects of black chokeberries, which have been widely cultivated on many farms in Korea. Using a 500 μg/mL concentration of a 70% ethanol extract from black chokeberries, we observed approximately 77% electron donating ability, approximately 88% 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammounium salt (ABTS) radical scavenging activity, approximately 71% hydrogen peroxide scavenging ability, and 0.45 reducing power. Using 200 μg/mL from the ethylacetate fraction, we observed approximately 90% ABTS radical scavenging ability, approximately 95% electron donating ability, 95% superoxide dismutase-like activity, and approximately 98% hydrogen peroxide scavenging activity. Furthermore, the 0.831 reducing power was a particularly meaningful result as compared with epigallocatechin-3-gallate (positive control). The results of a total phenolic content experiment showed a relatively high value of 45 mg tannic acid equivalents/g, and the result of an astringent effect experiment also showed a relatively high effect at 5000 μg/mL, with about 85% of the astringent effect in the ethylacetate fraction and 76% in the water fraction. The result of an elastase inhibition ability experiment showed a relatively high inhibition ability at 500 μg/mL; 76% of inhibition ability in the 70% ethanol extract and 74% of inhibition ability in the water fraction. Based on the study results, we determined that extracts and fractions from Korean black chokeberries have excellent anti-oxidant and anti-aging effects. Additionally, these results suggested that Korean black chokeberries may be used as a natural material in the cosmetic and functional food industries.
Published Version
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