Abstract
The health benefits of olive oil are well-known. In this study, the unsaponifiable fraction of extra virgin olive oil (Unsap) was investigated for reducing power capacity, ferric reducing antioxidant power, fer-rous chelating activity and nitric oxide inhibition. The present study was also designed to evaluate the in vitro cytotoxic effect of the Unsap against human carcinoma cells. The anti-inflammatory potential of Unsap has been determined via the inhibition of Human Cyclooxygenases. The results showed that Unsap is efficient for ferric reducing antioxidant power and nitric oxide inhibition. Unsap has a selective effect as anti-inflammatory agent. The results showed moderate to good in vitro antitumor activities of Unsap against human liver, lung and pancreas cancer cells with IC50 ranging from 19.6 to 30.4 μg/mL and good selectivity index (≥ 2). In conclusion, Unsap represents a promising and safe antitumor and antioxidant material that supports the need for further investigation.
Highlights
The results showed that unsaponifiable fraction of extra virgin olive oil (Unsap) is efficient for ferric reducing antioxidant power and nitric oxide inhibition
Squalene represented the main component of the Unsap while β-sitosterol, and γ-tocopherol were found in minor quantities as reported previously (Cardeno et al, 2014; Galal et al, 2020)
Previous studies indicated that the antioxidant activity of some compounds is correlated with their reducing power
Summary
EVOO was obtained from the Agriculture Research Center, Giza, Egypt. Ascorbic acid, butylated hydroxytoluene (BHT), MTT and trypan blue dye were purchased from Sigma (St. Louis, MO, USA). Fetal Bovine serum, DMEM, L-glutamine, gentamycin and 0.25% Trypsin-EDTA were purchased from Lonza (Walkersville, USA). Cell lines were obtained from the VACSERA Tissue Culture Unit, the Holding Company for Biological Products and Vaccines, Egypt
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