Abstract
An optimized enzymatic extraction using protamexTM was established for polysaccharides from Liparis tesellatus eggs (PLE). Total sugars, sulfated glycosaminoglycans, uronic acids, and hexosamines content of PLE were 43.2%, 9.0%, 23.9%, and 1.73%, respectively. Superoxide radical (SOx*) scavenging activity and 2,2-diphenyl-1-picrylhydrazyl (DPPH*) scavenging activity of PLE was tested to proof its antioxidant properties. Results on SOx* scavenging activity revealed that PLE has antioxidant activity although it’s not surpass the activity of Ascorbic acid as standard. DPPH* scavenging activity showed that PLE has activity increasing at a concentration-dependent manner. However, at 1.25 mg/ml, the anti-DPPH activities of PLE lowered, possibly due to the pro-oxidant action of the sample. PLE also has effects on NO production of RAW 264.7 cells, suggestive of their possible immunostimulating potential in the human body. On human keratinocyte (HaCaT cell line), HT29 and AGS cells lines, PLE was proofed has no toxicity and showed proliferative effects. This effect showed that PLE could have potency as wound healing promoter. An inference can be made that, PLE has indispensable antioxidant potencies as well as cell proliferating activities on specific cell lines which are deemed necessary in the field of medicine and their utilization as functional food supplements. Keywords: Antioxidant, Fish eggs, Immunostimulator, Liparis tessellatus, Polysaccharides
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.