Antioxidant and Free Radical Scavenging Effects of Lippia nodiflora

Abstract

This study evaluated the in vitro antioxidant potential of methanol extract of Lippia nodiflora Mich. (Verbenaceae) (MELN). The different antioxidants assays, including total antioxidant activity, reducing power, free radical, superoxide anion radical, hydroxyl radical, hydrogen peroxide, nitric oxide scavenging, and total phenolic content, were studied. MELN exhibited potent total antioxidant activity that increased with increasing amount of extract concentration (50, 100, 200, and 400 μg/mL), which were compared with standard drug α -tocopherol (400 μg/mL). The different concentrations of MELN and α -tocopherol showed inhibition of 49.07%, 58.96%, 63.07%, 68.29%, and 74.59%, respectively, on peroxidation in linoleic acid emulsion. In addition, MELN had effective reducing power, free radical scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging, hydrogen peroxide radical scavenging, and nitric oxide scavenging activity, and total phenolic content depending on concentration. These various antioxidant activities were compared with standard antioxidants such as BHA, BHT, catechin, and α -tocopherol.