Abstract
This study aims to evaluate the antioxidant and cytoprotective activities of 32 fungal strains isolated from brown seaweeds collected from Nha Trang Bay, Khanh Hoa Province, Central Vietnam. These fungal extracts were screened for antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical scavenging assay. In contrast, cytoprotective activity on rat cardiomyocytes H9c2 cell line was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. The results indicated the capacity for free DPPH and ABTS radical scavenging of fungal crude extracts with 31.25% (n = 10) and 81.25% (n = 26), respectively. Among the fungal strains with high antioxidant activity, the three fungal extracts 2104NT-1.3, 2104NT-3.3, and 2104NT-7.7 increased the viability of rotenone-exposed cardiomyocyte cells by 9.9%, 15.2%, and 13.6%, respectively. Three fungal strains with significant antioxidant and cytoprotective activities were identified as Penicillium chermesinum 2104NT-1.3, Aspergillus sp. 2104NT-3.3, and Penicillium sp. 2104NT-7.7 based on sequence analysis of internal transcribed spacer (ITS) region. This study provided the potential fungal strains isolated from Nha Trang Bay for further investigation of antioxidant and cytoprotective compounds.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
