Antioxidant and Antidiabetic Activities of Mempening (Lithocarpus bancanus) Leaves

Muhammad Almurdani,Titania T Nugroho,Yum Eryanti,Rudi Hendra,Jasril Karim,Adel Zamri,Hilwan Yuda Teruna

doi:10.5530/pj.2020.12.52

Abstract

Background: Lithocarpus bancanus or commonly called as mempening in Talang Mamak Tribe, Indonesia is a plant that is used as a traditional medicine. Objective: This study aim to evaluated antioxidant and antidiabetic activities of L. bancanus leaves extract. Material and Methods: The methanol extract was obtained by maceration of the leaves. The n-hexane, dichloromethane and ethyl acetate fractions were prepared by successive partition process of the methanol extract. Antioxidant activities were evaluated by various antioxidant assays, including DPPH (1,1-diphenyl-2-picrylhydrazyl), FRAP (ferric reducing antioxidant power), CUPRAC (cupric reducing antioxidant capacity), and ABTS (2,2’-azonobis 3-ethylbenzothiazoline-6-sulfonic acid) method. Total phenolics were estimated based on the Folin–Ciocalteu method, while, aluminum chloride methods were employed to estimate total flavonoids. Antidiabetic activies was determined by inhibiting the activity of α-glucosidase method. Results: antioxidant activity assay against DPPH radical as well as the total phenolic and flavonoid content of L. bancanus leaves showed that the methanol extract possessed IC50 value of 39.469 ± 0.273 μg/mL with total phenol and flavonoid were 11.426 ± 0.432 mg GAE/g dry weight sample and 15.423 ± 0.213 mg QE/g respectively. The FRAP, CUPRAC and ABTS values of methanol extract were 3494.302 ±0.456, 26665.501 ± 5.940 and 2857.977 ± 0.715 μM TE/g dry weight sample respectively. Antidiabetic activity of methanol extract with IC50 value of 30.565 ± 0.331 μg/mL. Conclusion: It could be concluded that leaves of L. bancanus have antioxidant and antidiabetic properties.

Highlights

Diabetes mellitus is the common serious metabolic disorder due to disturbance of carbohydrate, lipid and protein metabolism
Results: antioxidant activity assay against DPPH radical as well as the total phenolic and flavonoid content of L. bancanus leaves showed that the methanol extract possessed IC50 value of 39.469 ± 0.273 μg/mL with total phenol and flavonoid were 11.426 ± 0.432 mg GAE/g dry weight sample and 15.423 ± 0.213 mg QE/g respectively
The development of diabetes and progression of complications are usually associated with oxidative stress which is as a result of overexpression of reactive oxygen species (ROS) or free radicals

Summary

INTRODUCTION

Diabetes mellitus is the common serious metabolic disorder due to disturbance of carbohydrate, lipid and protein metabolism. 90% of all cases of diabetes in developed and developing countries are type-2 diabetes.[1,2] Hyperglycemia is found to increase the production of free radicals that are associated with long-term damage, dysfunction, and failure of various organs, especially eyes, kidneys, nerves, hearts, and blood vessels Several other factors such as hyperlipidaemia and enhanced oxidative stress play a major role in diabetes. Considering the high Indonesia biodiversity, it is essential to explore potential plant species, including L. bancanus (mempening) This species belongs to Fagaceae family found in the Talang Mamak tribe in Kelayang District, Indragiri Hulu Regency, Riau Province. It is usually used by the peoples as a medicine to treat pain and inflammation. Bioactivities from the genus have been evaluated including antioxidants, antidiabetic, anticancer, antimicrobial and other activities.[5,6,7,8] In regard to explore antidiabetic agent from Talang Mamak medicinal plants[9], we reported the antioxidant and antidiabetic activity of L. bancanus leaves extract and fractions

MATERIAL AND METHOD

AND DISCUSSION

Findings

CONCLUSION