Anti-oxidant and anti-diabetes activities of agarwood extracts from Gyrinops versteegii (Gilg.) Domke and their cytotoxicity

Abstract

Gyrinops versteegii is an endemic plant in Lombok, West Nusa Tenggara, which produce agarwood. In vitro anti-oxidant and anti-diabetes activities from several extracts of agarwood produced by G. versteegii were investigated. The 1, 1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging activity assay was used to determine the anti-oxidant activity, whereas the α-glucosidase inhibitory activity assay was conducted to determine the anti-diabetes activity. The toxicity test using brine shrimp lethality test (BSLT) and phytochemical analysis were also conducted in this experiment. As the results, the acetone extracts of infected wood of G. versteegii had the highest anti-oxidant activity with the IC50 value of 65.62 μg/mL, whereas the acetone extracts of agarwood had the α-glucosidase inhibitory activity with the IC50 value of 53.46 μg/mL. The toxicity test to the both samples shows the high toxicity with the LC50 values of 1.98 and 15.44 μg/mL, respectively. The high anti-oxidant and anti-diabetes activities in both of the acetone extracts of infected wood and agarwood may be caused by their high content of total phenolic (12.75 and 9.17%, respectively) and total flavonoid (19.96 and 14.34%, respectively). The anti-diabetes activity found in agarwood extracts produced by G. versteegii from Lombok is firstly reported.