Abstract
Antioxidant and anticancer activity test on Leukemia cells L1210 were carried out on four fractions (ethanol extract, n-hexane, chloroform, and water fraction) of Eleutherine bulbosa [Mill.] Urb. The purpose of this research is to obtain IC50 antioxidant and anticancer activity of the active fraction of Eleutherine. bulbosa [Mill.] Urb on Leukemia cells L1210. The research method includes antioxidant activity test with DPPH (2,2-diphenyl-1-picrylhydrazyl) method, anticancer activity test of active fraction on Leukemia cell L1210, and GC-MS analysis to determine the active compound. Chloroform fraction has the best antioxidant activity with IC50 value 19.694 ppm (very strong category). The IC50 value of the chloroform fraction on L1210 Leukemia cells was 9.56 ppm (very strong category). Based on GC-MS analysis of the chloroform fraction, some compounds such as hexadecanoic acid, 9,12-octadecadienoic acid, linolenic acid, octadecanoic acid, androstan-17-one, and 1-(2,3,5,6-tetramethylphenyl)- ethanone were detected.
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