Abstract
A potent anti-inflammatory compound was isolated and identified from leaves of Ligularia fischeri. The ethyl acetate fraction was used for purification of the compound and yielded 3,5-dicaffeoylquinic acid (DCQA). The identity of the compound was confirmed using 1H, 13C NMR, and MS analysis. DCQA showed strong DPPH (IC50=4.26 μg/mL), ABTS (TEAC value=0.9974) radical scavenging, and FRAP activities (=3.84 mmole of Trolox equivalent/g). DCQA effectively inhibited production of NO in RAW 264.7 cells induced using lipopolysaccharide (LPS) in a concentration dependent manner (50–250 μg/mL). DCQA significantly suppressed (p<0.05) up-regulation of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and tumor necrosis factor-α (TNF-α) gene expressions. The isolated DCQA can be useful for development of effective anti-inflammatory agents.
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