Effects of hypothermia resuscitation on expression of peroxisome proliferator activated receptor gamma, inducible nitric oxide synthase and tumor necrosis factor-α genes in lung tissues of rats with hemorrhagic shock

Abstract

Objective To investigate the effects of different body temperature resuscitation on the expression of peroxisome proliferator activated receptor gamma (PPAR-γ),inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α (TNF-α) genes in the lung tissue of rats with hemorrhagic shock.Methods Thirty-six adult male Wistar rats were randomly assigned to three groups:control group (S),normal thermia group (NR) and hypothermia group (HR).Rats in S control group underwent the surgical procedure but were not bled.Rats in NR group and HR group received normal thermia (37-38 ℃) resuscitation or hypothermia (33-34 ℃) resuscitation respectively during resuscitation and hourly thereafter.Realtime polymerase chain reaction (teal-time PCR) method was used to detect the mRNA expression of PPAR-γ,iNOS and TNF-α in the lung tissutes of rats with hemorrhagic shock after resuscitation for 60 min or 240min.Results (1) As compared with S group,PPAR-γ mRNA expression in NR group and HR group was0.61 ± 0.09 and 1.71 ± 0.16 respectively (P ＜ 0.05) after resuscitation for 60 min following hemorrhagic shock and 0.54 ± 0.08,and 2.82 ± 0.19 (P ＜ 0.05) after resuscitation for 240 min following hemorrhagic shock ; (2) As compared with S group,iNOS mRNA expression in NR group and HR group was 3.43 ±0.27 and 2.21 ±0.28 (P ＜0.05) after resuscitation for 60 min following hemorrhagic shock,and 4.86 ±0.62 and 2.93 ± 0.37 (P ＜ 0.05) after resuscitation for 240 min following hemorrhagic shock; (3) As compared with S group,TNF-α mRNA expression in NR group and HR group was 4.12 ±0.30 and 3.36 ±0.34 (P ＜0.05) after resuscitation for 60 min following hemorrhagic shock,and 5.87 ±0.34 and 4.91 ±0.53 (P ＜ 0.05) after resuscitation for 240 min following hemorrhagic shock.Conclusion Hypothermia resuscitation after hemorrhagic shock can raise the PPAR-γgene expression and inhibit iNOS and TNF-α gene expression in the rat lung tissues.Hypothermia resuscitation can reduce the lung tissue damage. Key words: Hemorrhagic shock; Hypothermia resuscitation; Peroxisome proliferator activated receptor gamma; Inducible nitric oxide synthase; Tumor necrosis factor-α