Abstract
ABSTRACTAntioxidant activity of aqueous extracts from two cultivated strawberry leaves, Senga Sengana and Mount Everest, obtained by maceration, reflux extraction and ultrasonic extraction, was investigated. Antioxidant activity of extracts was assessed by DPPH, FRAP, FIC and H2O2 test; total phenolics and flavonoids were assessed by Folin–Ciocalteu and aluminium chloride methods, respectively. Extract obtained from Senga Sengana by reflux extraction, containing higher amounts of extractive matter, total phenolics and flavonoids, has shown higher antioxidant activity (EC50(DPPH) = 10.4 μg/mL; FRAP = 258.61 mgFe2+/g dry extract.; FIC activity = 62.6% in concentration of 2 mg/mL; H2O2 scavenging = 25.43% in concentration of 0.5 mg/mL) compared to extracts obtained by other extraction techniques. Rutin, coumaroyl-galloyl-glucopyranoside, hydroxyphenylethyl-coumaroyl-glucopyranoside, derivates of hydroxyphenylethyl-coumaroyl-glucopyranoside, chlorogenic acid and p-coumaroylhexose have been identified by UHPLC–DAD–HESI–MS analysis.
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