Antioxidant activity of Galium mollugo L. extracts obtained by different recovery techniques

Petar Milic,Ljubisa Nikolic,Slavica Milic,Vlada Veljkovic,Vesna Nikolic,Katarina Rajkovic,Ljiljana Stanojevic

doi:10.2298/hemind120314042m

Abstract

The yield of extractive substances, antioxidant activity, as well as total phenolic and total flavonoid contents of aqueous-ethanolic extracts obtained from aerial parts of Galium mollugo L. by different extraction techniques (maceration, reflux and ultrasonic extraction) were reported. The antioxidant activity of extracts was tested by measuring their ability to scavenge a stable DPPH free radical, while the total phenolic and total flavonoid contents were determined according to the Folin-Ciocalteu procedure and a colorimetric method, respectively. The Duncan?s multiple range tests were used to evaluate if there were significant differences among yields of extractive substances, total phenolics, total flavonoids and EC50 values for the extracts obtained by different extraction techniques. The extracts obtained by the reflux extraction contained higher amounts of extractive substances, as well as phenolic and flavonoid compounds, and showed a better antioxidant activity than those obtained by the two other recovering techniques.

Highlights

The yield of extractive substances, antioxidant activity, as well as total phenolic and total flavonoid contents of aqueous-ethanolic extracts obtained from aerial parts of Galium mollugo L. by different extraction techniques are reported
According to the results of Duncan's multiple range test at the 95% significance level, the yield of extractive substances achieved by the reflux extraction was only significantly higher by approximately 5% than that obtained by the maceration, which was attributed to the higher extraction temperature
A comparative study of antioxidant activity, content of total phenolic and total flavonoid compounds of the aqueous-ethanolic extracts obtained from aerial parts G. mollugo by extraction in the presence and the absence of ultrasound was carried out

Summary

Introduction

The yield of extractive substances, antioxidant activity, as well as total phenolic and total flavonoid contents of aqueous-ethanolic extracts obtained from aerial parts of Galium mollugo L. by different extraction techniques (maceration, reflux and ultrasonic extraction) are reported. The antioxidant activity of extracts was tested by measuring their ability to scavenge a stable DPPH free radical, while the total phenolic and total flavonoid contents were determined according to the Folin–Ciocalteu procedure and a colorimetric method, respectively. Due to an ever increasing number of malignant, cardio-vascular diseases and infections, in recent times, the attention is drawn to plants with antioxidative activity. These disorders are considered to be provoked, in most cases, by harmful effects of free radicals [1,2]. The effect of ultrasound on the kinetics and the yield of extractive substances from the plant material is hard to predict because it depends on many factors such as the nature of

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