Antioxidant Activity of Secondary Metabolite Compounds from Lichen Teloschistes flavicans

Abstract

In the last decade, lichen has become a research interest related to exploring secondary metabolites compounds. Lichen is a unique organism and contains specific chemical compounds rarely found in other natural sources; one species is the lichen Teloschistes flavicans. The objective study explores the toxicity effects and antioxidants activity properties from lichen Teloschistes flavicans isolate. The isolation of secondary metabolites was carried out by extraction, separation, and purification by means of gravity column chromatography, thin-layer chromatography, and purity tests. Toxicity activity test used the Mayer method by looking at the LC50 value, while the antioxidant activity test used the DPPH method by looking at the IC50 value. The results of the isolation obtained a needle-shaped crystal with the name of the compound is 3-[1'-(2",3"-dihydroxy-phenyl)-propyl]-7-hydroxy-chroman-4-one and the molecular formula C18H18O5. The toxicity test showed that extract and isolate compounds have LC50 values of 9.38 and 162.18 µg/mL, indicating that the isolated compound was toxic. The antioxidant test showed that the extract and isolated compounds have antioxidant activity with IC50 values of 54.05 and 127.38. The results of these studies provide scientific development of natural compounds that have the potential to act as antioxidants for diseases in the human body.