Antioxidant Activity of Salad Vegetables Grown in Korea

Abstract

The antioxidant activity of forty two kinds of salad vegetables grown in Korea was evaluated. Methanol extract of freeze-dried vegetable was assayed by radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Fe^(2+)-catalyzed lipid peroxidation inhibition by TBA method. Total phenolics were determined colorimetrically using Folin-Ciocalteu reagent. The highest radical scavenging activity was expressed by perilla leaf, followed by dandelion leaf, red and green leafy lettuce, of which IC50 was less than 0.10 mg/mL. Angelica leaf showed the highest inhibitory action for lipid peroxidation with 95%, and then dandelion leaf, water spinach, and perilla leaf inhibited over 80%. However, lettuce (Iceberg) and young Chinese cabbage exhibited the lowest antioxidant activity based on both assay methods. Highly positive correlations between antioxidative activities and total phenolics were observed (p＜0.001). The results suggested that salad vegetables, especially perilla leaf, leafy lettuce, dandelion or angelica, could be used for easily accessible sources of natural antioxidants.