Abstract
Hibiscus sabdariffa has gained attention for its antioxidant activity. There are many accessions of H. sabdariffa in the world. However, information on the quantification of antioxidant compounds in different accessions is rather limited. In this paper, a liquid chromatography/quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS) method for simultaneous determination of five antioxidant compounds (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, and isoquercitrin) in H. sabdariffa leaves was developed. The method was validated for linearity, sensitivity, precision, repeatability and accuracy. The validated method has been successfully applied for determination of the five analytes in eight accessions of H. sabdariffa. The eight accessions of H. sabdariffa were evaluated for their antioxidant activities by DPPH free radical scavenging assay. The investigated accessions of H. sabdariffa were rich in rutin and exhibited strong antioxidant activity. The two accessions showing the highest antioxidant activities were from Cuba (No. 2) and Taiwan (No. 5). The results indicated that H. sabdariffa leaves could be considered as a potential antioxidant source for the food industry. The developed LC-Q-TOF-MS method is helpful for quality control of H. sabdariffa.
Highlights
Hibiscus sabdariffa L. is an annual herb shrub popularly known as Roselle or Sorrel [1]
limits of detection (LOD) and limits of quantification (LOQ) values were less than 0.09 μg/mL and
These results indicated that H. sabdariffa leaves could be considered as a potential antioxidant source for the food industry
Summary
Hibiscus sabdariffa L. (family: Malvacea) is an annual herb shrub popularly known as Roselle or Sorrel [1]. (family: Malvacea) is an annual herb shrub popularly known as Roselle or Sorrel [1]. The plant is commonly used as beverages and folk medicines [2]. Many H. sabdariffa accessions (samples of a crop variety collected at a specific location and time) are widely cultivated in tropical and subtropical countries. In Africa, the leaves of H. sabdariffa are usually consumed as vegetables in the preparation of soups and sauces [3]. The leaf extract has been found to possess many bioactive properties, such as anti-oxidant [1,4], anti-tumor [5], anti-hyperammonemic [4], anti-atherosclerotic [3], anti-filarial [6]. The leaves of H. sabdariffa are rich in phenolics, which could be responsible for the antioxidant capacity. Yields of H. sabdariffa leaves may be about 10 t/ha
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