Abstract
Ethyl acetate and diethyl ether extracts, previously extracted with methanol, were obtained from flesh and skin of Eptatretus burgeri (hag fish) and Enedrias nebulosus (white spotted eel). Eight different extracts (4 mg/mL) were tested for DPPH free radical scavenging activity and the values were compared with commercial antioxidants (α-tocopherol and BHT). All extracts of E. burgeri exhibited significantly positive results (> 65%) in scavenging DPPH radicals compared to E. nebulosus. Thus, only E. burgeri extracts were tested for dose effect, hydrogen peroxide, hydroxyl radical, superoxide scavenging assays and heat stability at 25, 50, 75 and 100 °C for 30 min. All extracts of E. burgeri showed higher DPPH radical scavenging activities by increasing concentration. Significantly higher results were observed for hydroxyl radical scavenging activity when compared with commercial antioxidants. Relatively moderate activity and very low activity were exhibited for superoxide scavenging and hydrogen peroxide activities, respectively. Diethyl ether extracts of E. burgeri were stable with increased temperature, while ethyl acetate extracts were stable up to 75 °C. Thus, there is a high potential for E. burgeri being rich with heat-stable antioxidants that can scavenge hydroxyl radicals.
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