Abstract
Turmeric rhizome ( Curcuma domestica Val) and Trengguli bark ( Cassia fistula L) contain antioxidant compounds which can be determined by 1,1-Diphenyl-2- Pycrylhydrazyl (DPPH) free radical inhibition method. This research was conducted to determine DPPH free radical inhibition by ethanol extract of turmeric rhizome, the ethanol extract of trengguli bark, and a combination of turmeric rhizome extract - trengguli bark extract (1:1,5) with ascorbic acid as a comparison. Identification of secondary metabolite classes is performed by phytochemical screening. Antioxidant activity was performed by inhibition of free radical color of DPPH using UV-Vis spectrophotometry. The study showed IC 50 value of ascorbic acid, as a comparasion, is 3,14 μg/mL. While ethanol extract of trengguli bark has the best antioxidant activity with IC 50 value 10,98 μg/mL compare to combination ethanol extract of turmeric rhizome - trengguli bark (1 :1,5) and ethanol extract of turmeric rhizome with IC 50 value is 13,70 μg/mL and 41,95 μg/mL, respectively. K eywords: Antioxidant, Curcuma domestica, Cassia fistula, DPPH Method
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