Decrease Expression of Tumor Necrosis Factor - Alpha (TNF - α) and Sperm Count Increase in Type 1 Diabetes Mellitus Rat (Rattus norvegicus) Model with Turmeric Rhizome (Curcuma longa L) Extract

Abstract

Diabetes mellitus type 1 (DM type 1) is a type of diabetes caused by pancreatic β cells damaged which producing insulin. Animal DM Type 1 caused inflammation of the pancreas and its impact on the male reproductive organs may decrease the quality and quantity of sperm. This research was aimed to determine the effect of turmeric rhizome ethanol extract (Curcuma longa L.) based on sperm count and expression of tumor necrosis factor-alpha (TNF-α) in the pancreas organ. This experiment was designed by Completely Randomized Design. Induction of DM Type 1 in rats was conducted by injecting Multiple Low Dosage - Streptozotocin (MLD-STZ) intraperitoneally (i.p) at the dosage of 20 mg/Kg BW for 5 days. This study used male rats (Rattus norvegicus) aged 3 months Wistar strain which divided into 5 groups: negative control (A), DM Type 1 (B), DM Type 1 treated with turmeric rhizome Extract 1,2 g/kg BW (C), 1,8 g/kg BW (D) and 2,7 g/kg BW (E) for 42 days. Each group consisted of 4 rats. spermatozoa counted with hemocytometer and measurement of TNF-α expression used immunohistochemical methods. The result of the analysis on one way ANOVA (α = 0,05) data showed that spermatozoa count was significantly increased and TNF-α expression was decreased in rats which treated with turmeric rhizome ethanol extract 2,7 g/kg BW. This result indicates that turmeric rhizome extract may increase the number of spermatozoa and decrease the expression of TNF-α of DM Type 1 rats.