ANTIOXIDANT ACTIVITY OF ALKALOID FRACTIONS AND COMPOUNDS FROM Litsea cubeba Lour. Fruits

Abstract

Maceration was used to extract the fruit of Litsea cubeba (Lour.). To get alkaloid fractions, ethanol extract was fractionated by liquid-liquid extraction using n-hexane, and chloroform at pH 3, 7, 9, and 11. Preparative thin layer chromatography was used to isolate alkaloid compounds. The ABTS test was used to assess the antioxidant activity of fractions and isolates. Chloroform fractions at pH 7 and compound III had the highest IC50. They IC50 were 80.47 ± 0.19 and 17.73 ± 0.04 µg/mL. Compound III was showed maximum absorbance at (279.0; 227.0; 212.5 nm) and wave number (3394.72; 2943.37; 2839.22; 1599.34; 1462.04; 1369.46; 1319.31; 1111.00; 817.82 cm-1). This indicates that it is an alkaloid compound containing hydroxyl, methyl, methylene, and methoxy groups. The findings show that the alkaloid fractions and components of Litsea cubeba fruit have a high antioxidant potential.