Antioxidant activity and phytochemical compositions of Mucuna pruriens L. in different conditions of time and temperature extraction

Abstract

Mucuna pruriens L. is a type of legume with high total phenolic and flavonoid compounds and may be a source of antioxidants. Bioactive and chemical components contained in velvet beans were obtained through maceration. Temperature and time of extraction were needed to obtain the optimal extract. The present study aims to explore temperature (30-60°C) and time (120-360 minutes) of the extraction effects on total phenol compounds (TPC), total flavonoid compounds (TFC), total tannin compounds (TTC), and the antioxidant capacity: 1, 1-diphenyl-2-picrylhydrazyl radical - scavenging activity (DPPH-RSA), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid radical - scavenging activity (ABTS+-RSA), and ferric reducing antioxidant power (FRAP) of velvet beans using double factor experiments approach. All of the studies extraction conditions indicated a significant effect (p < 0.05) on yield, TPC, TFC, TTC, DPPH-RSA, ABTS+-RSA, and FRAP. These results pointed that velvet beans extract for 60°C at 360 minutes was the highest content of TPC, TFC, and TTC were obtained with values of 41.78 mg GAE/g, 309,65 mg QE/g, and 237,13 mg TAE/g. The antioxidant capacity was 38.15 mg AAEAC/g on DPPH-RSA, 6.36 mg AAEAC/g on ABTS+-RSA, and 35.06 mM FE2+ /g on FRAP.