Antioxidant Activity and Mechanism of Avenanthramides: Double H+/e- Processes and Role of the Catechol, Guaiacyl, and Carboxyl Groups.

Abstract

Avenanthramides (AVAs), unique phenolic compounds in oats, have attracted increasing interest due to their health benefits. Eight representative AVAs were studied using the density functional theory (DFT) method to elucidate their antioxidant activity and mechanism. Preference of different mechanisms was evaluated based on thermodynamic descriptors involved in double (2H+/2e-) free radical scavenging reactions. It was found that the hydrogen atom transfer (HAT) mechanism is more favorable in the gas and benzene phases, while sequential proton loss electron transfer (SPLET) is preferred in polar media. The results suggest the feasibility of the double HAT and double SPLET mechanisms for 2s and c-series AVAs. The sequential triple proton loss double electron transfer (StPLdET) mechanism represents the dominant pathway in aqueous solution at physiological pH. In addition, the sequential proton loss hydrogen atom transfer (SPLHAT) mechanism provides an alternative pathway to trap free radicals. Results also revealed the important role of the catechol, guaiacyl, and carboxyl moieties.