Antioxidant activities of snakehead (Channa striata) fish skin: peptides hydrolysis using protease tp2 isolate from swamp plant silage

Ace Baehaki,Indah Widiastuti,Citra Nainggolan,Nuni Gofar

doi:10.5219/1264

Ace Baehaki, Indah Widiastuti + Show 2 more

Open Access

https://doi.org/10.5219/1264

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Abstract

The purpose of this research was to study the antioxidants activities of peptides from skin fish of snakehead (Channa striata), using hydrolysis of protease TP2 isolate from swamp plant silage. This research 5 treatments hydrolysis time (0, 30, 60, 90, 120 min, respectively), with two replicates, which included several stages of preparation and pre-treatment of the snakehead fish skin production of protease enzymes which were isolated from swamp water, preparation of protein hydrolysates, measurement of hydrolysis degrees, analysis of peptides content and analysis of the antioxidant activity. Results showed that the treatment had given a significant effect on the 5% level of the degree of hydrolysis production (13.98% – 27.08%), with peptides content of 2.73% – 3.78% and antioxidant activity (10.75% – 20.7%). The results of the degree of hydrolysis indicate that the longer the hydrolysis time, the percent degree of hydrolysis will increase. Peptide content and antioxidant activity were increased with increasing hydrolysis time.

Highlights

Snakehead fish in South Sumatra is generally used as basic ingredients of the typical Palembang food industry, namely pempek, kerupuk, and kemplang
This research 5 treatments hydrolysis time (0, 30, 60, 90, 120 min, respectively), with two replicates, which included several stages of preparation and pre-treatment of the snakehead fish skin production of protease enzymes which were isolated from swamp water, preparation of protein hydrolysates, measurement of hydrolysis degrees, analysis of peptides content and analysis of the antioxidant activity
Scientific hypothesis Protease from swamp plant silage isolates can be used for hydrolysis of snakehead fish skin (Channa striata) to produces peptide with antioxidant activity

Summary

Introduction

Snakehead fish in South Sumatra is generally used as basic ingredients of the typical Palembang food industry, namely pempek, kerupuk, and kemplang. The processing process produces waste, one of which is skin waste. Unused waste contains very important nutritional compounds such as protein content (collagen and keratin) and mineral composition (Moller et al, 2008). This waste has the potential to be used as a protein hydrolysate containing bioactive peptides. Several studies have shown that fish protein hydrolysates have functional properties as antihypertensive, anticancer, antimicrobial, and antioxidant. Bioactive peptides can be obtained by several methods of hydrolysis, namely hydrolysis with digestive enzymes and hydrolysis by proteolytic enzymes produced by microorganisms or plants (Korhonen, 2009)

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