Antinociceptive effect of methanol extract of Dalbergia sissoo leaves in mice

Md Abdul Mannan,Md Farhad Hossen Khan,Ambia Khatun

doi:10.1186/s12906-017-1565-y

Md Abdul Mannan, Md Farhad Hossen Khan + Show 1 more

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https://doi.org/10.1186/s12906-017-1565-y

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Abstract

BackgroundDalbergia sissoo DC. (Family: Fabaceae) is a medium to large deciduous tree, is locally called “shishu” in Bangladesh. It is used to treat sore throats, dysentery, syphilis, bronchitis, inflammations, infections, hernia, skin diseases, and gonorrhea. This study evaluated the antinociceptive effect of the methanol extract of D. sissoo leaves (MEDS) in mice.MethodsThe extract was assessed for antinociceptive activity using chemical and heat induced pain models such as hot plate, tail immersion, acetic acid-induced writhing, formalin, glutamate, and cinnamaldehyde test models in mice at the doses of 100, 200, and 400 mg/kg (p.o.) respectively. Morphine sulphate (5 mg/kg, i.p.) and diclofenac sodium (10 mg/kg, i.p.) were used as reference analgesic drugs. To confirm the possible involvement of opioid receptor in the central antinociceptive effect of MEDS, naloxone was used to antagonize the effect.ResultsMEDS demonstrated potent and dose-dependent antinociceptive activity in all the chemical and heat induced mice models (p < 0.001). The findings of this study indicate that the involvement of both peripheral and central antinociceptive mechanisms. The use of naloxone verified the association of opioid receptors in the central antinociceptive effect.ConclusionsThis study indicated the peripheral and central antinociceptive activity of the leaves of D. sissoo. These results support the traditional use of this plant in different painful conditions.

Highlights

Dalbergia sissoo DC. (Family: Fabaceae) is a medium to large deciduous tree, is locally called “shishu” in Bangladesh
Analysis of the possible mechanism of action of MEDS Involvement of opioid system The possible connection of the opioid system in the antinociceptive effect of MEDS was examined by injecting naloxone hydrochloride (2 mg/kg, i.p.), a nonselective opioid receptor antagonist, and 15 min prior to the administration of either morphine or MEDS (100, 200 and 400 mg/kg, p.o.)
Involvement of cyclic guanosine monophosphate pathway To investigate the possible involvement of cGMP pathway to the antinociceptive effect of MEDS, mice were pre-treated with methylene blue (20 mg/kg), a nonspecific inhibitor of nitric oxide/guanylyl cyclase, intraperitonially 15 min before the administration of diclofenac sodium or MEDS (100, 200 and 400 mg/kg, p.o.)

Summary

Introduction

Dalbergia sissoo DC. (Family: Fabaceae) is a medium to large deciduous tree, is locally called “shishu” in Bangladesh. It is used to treat sore throats, dysentery, syphilis, bronchitis, inflammations, infections, hernia, skin diseases, and gonorrhea. (Family: Fabaceae) is a medium to large deciduous tree, is commonly known as sisu, sheesham, tahli, tali, jag at different parts of the world and is locally called “shishu” in Bangladesh. It is usually used to treat sore throats, dysentery, syphilis, and gonorrhoea [1] It is used in conditions like blood purifier, leprosy, headaches, bronchitis, inflammations, infections, hernia, and skin diseases [2]. Juice of the leaves is useful for anthelmintic, eye and nose diseases. It used in scabies, burning sensation, scalding urine and digestive disorders [5,6,7,8].

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