ANTI-NK 2.1: AN ACTIVITY OF NZB ANTI-BALB/c SERUM

Abstract

Publisher Summary This chapter presents a study focusing on anti-NK 2.1. Anti-NK 1.1 was raised by immunizing BALB/c × C3H F-1 mice with CE spleen cells (SC). The same immunization schedule was used to raise NZB anti-BALB/c, that is, NZB/B1NJ mice were injected intraperitoneally with progressively greater doses of BALB/c SC at 2-week intervals. After the fourth immunization, the NZB mice were bled individually and their sera tested for C dependent depletion of NK activity. BALB/c SC were treated with antiserum and C or antiserum alone in a one-step lysis and then tested at 3 effector:target ratios on 51Cr-labeled YAC-1 target cells. The sera were also screened for autoantibody by dye exclusion assay on BALB/c SC. Sera with autoantibody or low titers of anti-NK activity were discarded and the remaining sera pooled. Sera were subsequently obtained on days 4 and 7 after further immunizations. Analysis of NZB anti-BALB/c serum revealed that antibodies to NK-associated antigens were indeed present but that the activity was not to an allele of NK1. The data suggests that this antiserum detects an NK-associated antigen provisionally designated NK 2.1, which is neither an allele of nor linked to NK 1.