Abstract
Volatile oil of Eupatorium adenophorum leaves was extracted and analyzed by gas chromatography–mass spectrometry (GC–MS). Sesquiterpenes were identified asmajor constituents in the essential oil. Four cadinene sesquiterpenes were isolated from ethyl acetate extract of leaves and identified spectroscopically. Chemical constituents were evaluated for antinemic activity against Meloidogyne incognita. Antinemic activity of volatile oil was in the range of LC50 133.7–189.2 µg ml−1. Methanolic concentrate exhibited maximum antinemic activity (LC50 93.7–162.4 µg ml−1) followed by hexane extract (LC50 99.8–127.2 µg ml−1). Among pure constituents, 5,6-dihydroxycadinan-3-ene-2,7-dione exhibited significant activity (LC50 151.8 µg ml−1).
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