Antimutagenic test of ethanol extract of temu giring (Curcuma heyneana) rhizome on erythrocyte cell of mouse in vivo

Abstract

Ichshanti S, Arianingrum R, Atun S. 2014. Antimutagenic test of ethanol extract of temu giring (Curcuma heyneana) rhizome on erythrocyte cell of mouse in vivo. Bioteknologi 11: 28-35. The purpose of this research was to determine the percentage of the antimutagenic activity of methanol extract from temu giring (Curcuma heyneana) rizhome on erythrocyte cell of mouse. This research was performed by using the method of micronucleous test with the treatment of the methanol extract of temu giring rizhome peroral and cyclophosphamide intraperitoneal on male mice of Balb-c groove having with the age of 6-7 weeks and the body weight of 30-40 g. The treatment was performed for 2 days. Then, in the second day, six hours after the second treatment of cyclophosphamide, all mice were sacrificed by the neck dislocation and dissected to take the bone marrow from the femoral bone. Furthermore, the bone marrow smear preparation was made to observe the number of micronucleus polychromatic cells erythrocytes (MNPCE). The used dosages of methanol extract of temu giring rizhome were 300 and 600 mg/kg bw. The toxical compound used as a positive control was cyclophosphamide with a dosage of 50 mg/kg bw. The results showed that the methanol extract of temu giring rizhome with the dosages of 300 and 600 mg/kg bw given by cyclophosphamide with a dosage of 50 mg/kg bw had an antimutagenic activity. The percentage of antimutagenic activity of the methanol extract of temu giring rizhome with the dosages of 300 and 600 mg/kg bw was 95.5%.