Abstract
Background: β-lactams and carbapenems are the major antibiotics used to treat gram-negative bacteria and non-fermenting bacilli. However, the increasing production of β-lactamase and carbapenemase limits the therapeutic options. Our study aims to determine the resistant phenotypes of these bacteria while describing their epidemiological aspect. Material and Methods: This was a cross-sectional study by consecutive enrollment from January 2018 to December 2019 at Sominé DOLO Hospital of Mopti, Mali. We performed manual method for bacteria culture, identification and antibiotics sensitivity testing. The antibiotics sensitivity testing was accessed by the diffusion method according to CA-SFM/EUCAST (“Comité de l’Antibiogramme de la Société Française de Microbiologie” / European Committee on Antimicrobial Susceptibility Testing) recommendations V1.0 february 2018 and V2.0 may 2019 2019 V.2.0. May recommendation released in 2019. Data were analyzed by software R 4.0.3 GUI 1.73 Catalina build (7892). Results: At all 904 samples were included in this study. Out of the 904 cultures, 297 sample (32.85%) were positive. The rates of enzymes production were as follow: Extended-spectrum ß-lactamase (ESBL) 56.42% (101/179), cepholosporinase hyperproduction (HCASE) 15.64% (28/179), cephalosporinase production (CASE) 6.14% (11/179), penicillinase hyperproduction (HP) 5.58% (10/179), carbapenemase production (CP) 6.14% (11/179) and savage strains 10.05% (18/179). Conclusion: Our data showed a high prevalence of resistance to β-lactamins and carbapenemes in gram-negative bacteria and non-fermenting bacillus bacilli. The A high level of β-lactamase and carbapenemase production by gram negative bacillius were also reported by others authors calls for the rational use of antibiotic in hospital setting.
Highlights
Gram-negative bacteria bacillus, most notably Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, are among the most important causes of serious hospital-acquired and community-onset bacterial infections in humans [1]
Material and Methods: This was a cross-sectional study by consecutive enrollment from January 2018 to December 2019 at Sominé DOLO Hospital of Mopti, Mali
The β-Lactam antibiotics are a major drug class used to treat serious community-onset or hospital-acquired infections caused by gram negative bacillus, resistance to these agents will continue to challenge clinical therapeutic choices [4]
Summary
Gram-negative bacteria bacillus , most notably Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, are among the most important causes of serious hospital-acquired and community-onset bacterial infections in humans [1]. The β-Lactam antibiotics are a major drug class used to treat serious community-onset or hospital-acquired infections caused by gram negative bacillus, resistance to these agents will continue to challenge clinical therapeutic choices [4]. In Gram-negative bacillus, these enzymes production remains the most important contributing factor to β-lactam resistance and these dissemination is alarming [2]. A few data concern antibiotic-drug-resistance is available in Mali, that is a middle and income country and that not have a real antimicrobial resistance policy. The objective of this study was to determine the resistant phenotypes of gram negative bacteria while describing their epidemiological aspect at Sominé DOLO hospital of Mopti and the prevalence of these bacillus
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