Abstract
Salmonallaeand Shigellae bacteria of the family Enterobacteriaceae has been implicated as the causal agent of many gastro-intestinal disorders in man and animal. To determine the antimicrobial resistant pattern and plasmid profile of different resistant Salmonellae and Shigellae bacteria in Kaduna metropolis, Kaduna state, Nigeria, a total of fifty eight (58) isolates of clinical were collected randomly from diarrheal patients in the metropolis and tested against ten (10) commonly used antimicrobial agents using the Kirby-Bauer disc diffusion method. Plasmid DNA was extracted using FosmidMAX DNA purification kit and separated by agarose gel electrophoresis. Results indicated a varying degree of resistance to the different drugs tested, however, all Salmonella and Shigella isolates were found to be sensitive to at least one of the ten (10) drugs tested. Augmentin, Amoxicillin and streptomycin were found to be most resisted with no effect on any of the isolates while Chloramphenicol was the most effective with 25mm±1.00 and 23mm±1.00 against Salmonella and Shigella respectively. Plasmid profile revealed 31% success, suggesting no correlation between presence of plasmid and antibiotic resistance in the isolates. It was concluded that isolates might have harboured the presence of multi-drug resistance in their chromosomal genes rather than in plasmids.
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