Abstract
The background: E. coli infection is a septicemic disease with an economic importance of poultry causing multiple lesions in broiler chickens. Recent reports described increased resistance of E. coli to many antimicrobial agents. This research work aimed to detect, identify and study antimicrobial resistance genes of E. coli isolated from broiler chickens in Upper Egypt. Methods: Three-hundreds samples, including 200 liver and 100 tracheal swabs, were collected from broiler chicken flocks at different localities in Assiut and El-Minya Governorates. Samples were subjected to isolation and phenotypic identification, serological typing, detection of sensitivity and resistance to antimicrobial drugs and determination of genes responsible for resistance to antimicrobial agents. Results and conclusion: revealed that E. coli could be isolated and phenotypically identified with a percent ratio 70% (210 from 300). Twenty-six out of 30 E. coli isolates were serologically identified. Determination of antimicrobial susceptibility and resistance patterns of E. coli isolates to variable antimicrobial drugs using standard disk diffusion and minimum inhibitory concentration (MIC) methods was done. Detection of antimicrobial resistance genes of 12 serologically identified E. coli isolates showed that ƒloR gene (Florfenicol resistance gene) was detected in 6 isolates and strA-strB gene was detected in 5 isolates, while aadA gene was detected in all 12 isolates. Both strA-strB and aadA are streptomycin resistance genes.
Highlights
Colibacillosis is a systemic disease of poultry caused by avian pathogenic E. coli (APEC) [15] and characterized by septicemia with multiple organ lesions, typically pericarditis, air-sacculitis, perihepatitis, peritonitis, and other extraintestinal lesions
Approaches to control APEC infections in the poultry industry include improved hygienic methods, vaccination, use of competitive exclusion products, and the introduction of novel immunopotentiators each of these practices have had restricted success [55]. This has necessitated the use of antimicrobial chemotherapy to control outbreaks of colibacillosis, but modern reports have described increased resistance to those antimicrobial agents commonly used for treatment [85]
Detection of antibacterial resistance genes in 12 serologically identified E. coli isolates showed that ƒloR gene (Florfenicol resistance gene) was detected in 6 isolates and strA-strB gene was detected in 5 isolates, while aadA gene was detected in all 12 isolates. both strA-strB and aadA are streptomycin resistance gene
Summary
Colibacillosis is a systemic disease of poultry caused by avian pathogenic E. coli (APEC) [15] and characterized by septicemia with multiple organ lesions, typically pericarditis, air-sacculitis, perihepatitis, peritonitis, and other extraintestinal lesions. Approaches to control APEC infections in the poultry industry include improved hygienic methods, vaccination, use of competitive exclusion products, and the introduction of novel immunopotentiators each of these practices have had restricted success [55]. This has necessitated the use of antimicrobial chemotherapy to control outbreaks of colibacillosis, but modern reports have described increased resistance to those antimicrobial agents commonly used for treatment [85]. Multi-drug resistant strains of E. coli are ever-present in both human and animal isolates in different parts of the world [8] and multiple drug resistant, nonpathogenic E. coli found in the intestine is maybe an important reservoir of resistance genes [74] and momentarily drug-resistant E. coli of animal origin may colonize the human
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