Abstract
Abstract Nanoparticles exhibit unique physiochemical properties leading to their potential applications in the field of biotechnology, electronics and drug delivery. The synthesis of uniform nanosized particles with specific properties related to size, shape, and physical and chemical properties is of great interest for different applications. The bacterial resistance towards conventional antibiotics has increased over time and development of new antibacterial agents is required to fight the pathogenic bacteria. Metallic nanoparticles are safe, durable and heat resistance compared to the conventional antibacterial agents. In the present work, silver (Ag), gold (Au) and zinc oxide (ZnO) nanoparticles have been synthesized via polyol and precipitation methods. The nanoparticles have been characterized by UV-Visible Spectroscopy and X-ray Diffraction studies. The size and morphology of the synthesized nanoparticles has been studied using FESEM analysis. The antibacterial activity of these nanoparticles was investigated qualitatively against Escherichia coli (E. coli) bacteria as a model for a pathogenic gram-negative bacterium. Bacteriological tests were performed in Luria–Bertani (LB) liquid medium and on solid agar plates using UV-Visible spectroscopy. The nanoparticles show enhanced antibacterial activities and potential application towards realization of bactericides.
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