Abstract
Background: It has been demonstrated that azole-resistant strains of Candida albicans have a greater resistance to antimicrobial photodynamic therapy (aPDT) when compared to their more susceptible counterparts. For this reason, the present study evaluated the efficacy of aPDT, together with nystatin (NYS), in the treatment of oral candidiasis in vivo. Methods: Mice were infected with fluconazole-resistant C. albicans (ATCC 96901). To perform the combined therapy, aPDT, mediated by Photodithazine (PDZ) and LED light, was used together with NYS. The efficacy of the treatments was evaluated by microbiological, macroscopic, histopathological and Confocal Scanning Laser Microscopy analyses of the lesions. The expression of p21 and p53, proteins associated with cell death, from the tongues of mice, was also performed. Results: The combined therapy reduced the fungal viability by around 2.6 log10 and decreased the oral lesions and the inflammatory reaction. Additionally, it stimulated the production of p53 and p21. Conclusions: The combined therapy is a promising alternative treatment for oral candidiasis induced by C. albicans resistant to fluconazole.
Highlights
Candida spp. are commensal constituents of the oral microbiota and gastrointestinal tract in healthy individuals [1]
The purpose of the present investigation was to evaluate the efficacy of antimicrobial photodynamic therapy (aPDT) mediated by PDZ, together with the antifungal nystatin, in the treatment of induced OC in mice infected with C. albicans resistant to fluconazole
The present investigation assessed the efficacy of aPDT, applied in tandem with nystatin, in the treatment of induced OC in mice infected with C. albicans resistant to fluconazole
Summary
Candida spp. are commensal constituents of the oral microbiota and gastrointestinal tract in healthy individuals [1]. A single session of aPDT mediated by PDZ reduced the cell viability of fluconazole-resistant C. albicans in mice with experimental oral candidosis by 1.96 log (OC) [16]. Based on the above-mentioned studies, aPDT mediated by PDZ demonstrated satisfactory results in the inactivation of Candida spp. and in the treatment of experimental OC [12,13,14,15,16,17]. It has been demonstrated that azole-resistant strains of Candida albicans have a greater resistance to antimicrobial photodynamic therapy (aPDT) when compared to their more susceptible counterparts For this reason, the present study evaluated the efficacy of aPDT, together with nystatin (NYS), in the treatment of oral candidiasis in vivo. Conclusions: The combined therapy is a promising alternative treatment for oral candidiasis induced by C. albicans resistant to fluconazole
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