Antimicrobial evaluation of hydroalcoholic extract and Volatile components of aerial part of Kelussia odoratissima Mozaff.

Abstract

Kelussia odoratissima Mozaff. is a sweet-smelling, wild plant which is traditionally consumed in Iran as a garnish. Only Little, is known about its potential antioxidant activity. In this study.The antibacterial activities. against Staphylococus aureus, Escherichia coli, Bacillus cereus and Pseudomonas aeruginosa were investigated by paper Disc and immersion bioautography Methods. Separately Ethanolic extract was taken by maceration method and Volatile components was taken by cold solvent extraction method using petroleum ether. Inhibitory zones of blank discs, impregnated with defined amounts of extract were observed. and MIC were measured using E-Test. method. In bioautographic Method, extract and volatile components were separated on silica gel TLC plates to the best resolution then were placed on agar plate inoculated with bacterial suspension. After incubation for 16–18 hr at 37°C, visualization of inhibition zones was done by spraying the surface of culture media with MTT solution. Clear zone in dark purple background was the positive answer. Another TLC sheet developed with the same solvent, in the same condition was sprayed with known spraying reagents commonly used for detecting known phytochemical groups. Hydroalcoholic extract of Kelussia odoratissima were effective against E. coli, B. cereus, P. aeruginosa and S. aureus with MIC (0.64, 0.25, 0.43, 0.43mg/disc) and volatile components on S. aureus. B. cereus with MIC (0.43, 0.18mg/disc).